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Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli

Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one codon” metho...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Kulmala, Antti, Huovinen, Tuomas, Lamminmäki, Urpo
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5476587/
https://ncbi.nlm.nih.gov/pubmed/28630449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03957-6
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