Production of recombinant human epidermal growth factor in Pichia pastoris

This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the m...

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Bibliographic Details
Published in:Braz J Microbiol
Main Authors: Eissazadeh, Samira, Moeini, Hassan, Dezfouli, Maryam Ghandizadeh, Heidary, Somayyeh, Nelofer, Rubina, Abdullah, Mohd Puad
Format: Artigo
Language:Inglês
Published: Elsevier 2017
Subjects:
Biotechnology and Industrial Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5470459/
https://ncbi.nlm.nih.gov/pubmed/27998673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bjm.2016.10.017
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