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Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies

Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach nitroxide spin labels to proteins and how to collec...

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Bibliografische gegevens
Gepubliceerd in:Bio Protoc
Hoofdauteurs: Sjodt, Megan, Clubb, Robert T.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-Protocol 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5467883/
https://ncbi.nlm.nih.gov/pubmed/28616445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2207
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