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Site-Directed Parallel Spin-Labeling and Paramagnetic Relaxation Enhancement in Structure Determination of Membrane Proteins by Solution NMR Spectroscopy

A major challenge for the structure determination of integral membrane proteins by solution NMR spectroscopy is the limited number of NOE restraints in these systems stemming from extensive deuteration. Paramagnetic relaxation enhancement (PRE) by means of nitroxide spin-labels can provide valuable...

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Détails bibliographiques
Auteurs principaux: Liang, Binyong, Bushweller, John H., Tamm, Lukas K.
Format: Artigo
Langue:Inglês
Publié: 2006
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3199951/
https://ncbi.nlm.nih.gov/pubmed/16569016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja0574825
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