Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice

Brain endothelial cells are the major building block of the blood-brain barrier. To study the role of brain endothelial cells in vitro, the isolation of primary cells is of critical value. Here, we describe a protocol in which vessel fragments are isolated from adult mice. After density centrifugati...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Assmann, Julian Christopher, Müller, Kristin, Wenzel, Jan, Walther, Thomas, Brands, Josefine, Thornton, Peter, Allan, Stuart M., Schwaninger, Markus
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Methods Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5464392/
https://ncbi.nlm.nih.gov/pubmed/28603749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2294
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