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Tautomeric stabilities of 4-fluorohistidine shed new light on mechanistic experiments with labeled ribonuclease A
Ribonuclease A is the oldest model for studying enzymatic mechanisms, yet questions remain about proton transfer within the active site. Seminal work by Jackson et al. (Science, 1994) labeled Ribonuclease A with 4-fluorohistidine, concluding that active-site histidines act as general acids and bases...
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發表在: | Chem Phys Lett |
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Main Authors: | , , , |
格式: | Artigo |
語言: | Inglês |
出版: |
2016
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5461937/ https://ncbi.nlm.nih.gov/pubmed/28603294 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cplett.2016.10.072 |
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