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Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell

BACKGROUND: Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperature...

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Detalhes bibliográficos
Main Authors: Hartshorn, Cristina, Anshelevich, Aleksandra, Wangh, Lawrence J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC546192/
https://ncbi.nlm.nih.gov/pubmed/15649321
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-5-2
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