Real-time analysis of the binding of fluorescent VEGF(165)a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF(165)a-TMR) labeled on a single cysteine within each protomer of the antiparallel VEGF homodimer. VEGF(1...

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Pubblicato in:Biochem Pharmacol
Autori principali: Kilpatrick, Laura E., Friedman-Ohana, Rachel, Alcobia, Diana C., Riching, Kristin, Peach, Chloe J., Wheal, Amanda J., Briddon, Stephen J., Robers, Matthew B., Zimmerman, Kris, Machleidt, Thomas, Wood, Keith V., Woolard, Jeanette, Hill, Stephen J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Science 2017
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5457915/
https://ncbi.nlm.nih.gov/pubmed/28392095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bcp.2017.04.006
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