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Mutants with Temperature-Sensitive Defects in the Escherichia coli Mismatch Repair System: Sensitivity to Mispairs Generated In Vivo

We have used direct selections to generate large numbers of mutants of Escherichia coli defective in the mismatch repair system and have screened these to identify mutants with temperature-sensitive defects. We detected and sequenced mutations that give rise to temperature-sensitive MutS, MutL, and...

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Detalhes bibliográficos
Main Authors: Hong, Esther S., Yeung, Annie, Funchain, Pauline, Slupska, Malgorzata M., Miller, Jeffrey H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC545721/
https://ncbi.nlm.nih.gov/pubmed/15659661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.3.840-846.2005
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