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Mutants with Temperature-Sensitive Defects in the Escherichia coli Mismatch Repair System: Sensitivity to Mispairs Generated In Vivo

We have used direct selections to generate large numbers of mutants of Escherichia coli defective in the mismatch repair system and have screened these to identify mutants with temperature-sensitive defects. We detected and sequenced mutations that give rise to temperature-sensitive MutS, MutL, and...

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Autores principales: Hong, Esther S., Yeung, Annie, Funchain, Pauline, Slupska, Malgorzata M., Miller, Jeffrey H.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC545721/
https://ncbi.nlm.nih.gov/pubmed/15659661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.3.840-846.2005
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