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Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU
Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExo...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology
2005
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC545705/ https://ncbi.nlm.nih.gov/pubmed/15659695 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.3.1192-1195.2005 |
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