Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU

Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Sato, Hiromi, Feix, Jimmy B., Hillard, Cecilia J., Frank, Dara W.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2005
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC545705/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15659695/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.187.3.1192-1195.2005
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