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Evaluation of vector-primed cDNA library production from microgram quantities of total RNA

cDNA sequences are important for defining the coding region of genes, and full-length cDNA clones have proven to be useful for investigation of the function of gene products. We produced cDNA libraries containing 3.5–5 × 10(5) primary transformants, starting with 5 μg of total RNA prepared from mous...

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Hlavní autoři: Kuo, Jonathan, Inman, Jason, Brownstein, Michael, Usdin, Ted B.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC545479/
https://ncbi.nlm.nih.gov/pubmed/15601994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh181
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