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Evaluation of vector-primed cDNA library production from microgram quantities of total RNA
cDNA sequences are important for defining the coding region of genes, and full-length cDNA clones have proven to be useful for investigation of the function of gene products. We produced cDNA libraries containing 3.5–5 × 10(5) primary transformants, starting with 5 μg of total RNA prepared from mous...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC545479/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15601994/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh181 |
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