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Single-protein detection in crowded molecular environments in cryo-EM images

We present an approach to study macromolecular assemblies by detecting component proteins’ characteristic high-resolution projection patterns, calculated from their known 3D structures, in single electron cryo-micrographs. Our method detects single apoferritin molecules in vitreous ice with high spe...

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Bibliographic Details
Published in:eLife
Main Authors: Rickgauer, J Peter, Grigorieff, Nikolaus, Denk, Winfried
Format: Artigo
Language:Inglês
Published: eLife Sciences Publications, Ltd 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5453696/
https://ncbi.nlm.nih.gov/pubmed/28467302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.25648
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