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Structure-Based Engineering of an Artificially Generated NADP(+)-Dependent d-Amino Acid Dehydrogenase

A stable NADP(+)-dependent d-amino acid dehydrogenase (DAADH) was recently created from Ureibacillus thermosphaericus meso-diaminopimelate dehydrogenase through site-directed mutagenesis. To produce a novel DAADH mutant with different substrate specificity, the crystal structure of apo-DAADH was det...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Hayashi, Junji, Seto, Tomonari, Akita, Hironaga, Watanabe, Masahiro, Hoshino, Tamotsu, Yoneda, Kazunari, Ohshima, Toshihisa, Sakuraba, Haruhiko
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5440713/
https://ncbi.nlm.nih.gov/pubmed/28363957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00491-17
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