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Structure-Based Engineering of an Artificially Generated NADP(+)-Dependent d-Amino Acid Dehydrogenase
A stable NADP(+)-dependent d-amino acid dehydrogenase (DAADH) was recently created from Ureibacillus thermosphaericus meso-diaminopimelate dehydrogenase through site-directed mutagenesis. To produce a novel DAADH mutant with different substrate specificity, the crystal structure of apo-DAADH was det...
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| 出版年: | Appl Environ Microbiol |
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| 主要な著者: | , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology
2017
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5440713/ https://ncbi.nlm.nih.gov/pubmed/28363957 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00491-17 |
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