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Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis
Urate oxidase is a key enzyme in purine metabolism and catalyzes the oxidation of uric acid to allantoin. It is used to treat hyperuricemia and gout, and also in a diagnostic kit. In this study, error-prone polymerase chain reaction and staggered extension process was used to generate a mutant urate...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5439685/ https://ncbi.nlm.nih.gov/pubmed/28531234 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0177877 |
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