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Simple, fast and high‐efficiency transformation system for directed evolution of cellulase in Bacillus subtilis
Bacillus subtilis can serve as a powerful platform for directed evolution, especially for secretory enzymes. However, cloning and transformation of a DNA mutant library in B. subtilis are not as easy as they are in Escherichia coli. For direct transformation of B. subtilis, here we developed a new p...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Publishing Ltd
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3815800/ https://ncbi.nlm.nih.gov/pubmed/21255377 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2010.00230.x |
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