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Highly specific imaging of mRNA in single cells by target RNA-initiated rolling circle amplification

Detection of single-cell gene expression with high spatial and sequence resolution is a key challenge in single cell biology. Herein, we propose a robust method for the direct detection of mRNA, termed target RNA-initiated rolling circle amplification, which enables imaging of mRNA with single-nucle...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Chem Sci
Main Authors: Deng, Ruijie, Zhang, Kaixiang, Sun, Yupeng, Ren, Xiaojun, Li, Jinghong
Format: Artigo
Jezik:Inglês
Izdano: Royal Society of Chemistry 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5437493/
https://ncbi.nlm.nih.gov/pubmed/28580104
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc00292k
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