Isolation of mutants of human immunodeficiency virus protease based on the toxicity of the enzyme in Escherichia coli.
The protease encoded by the pol gene of human immunodeficiency virus was expressed in Escherichia coli and found to be toxic to strain BL21(DE3). This toxicity provided a convenient selection for isolating mutants of the protease that are nontoxic and enzymatically inactive. This strong correlation...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54367/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2196572/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.14.5573 |
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