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Methyltransferase-directed covalent coupling of fluorophores to DNA
We report an assay for determining the number of fluorophores conjugated to single plasmid DNA molecules and apply this to compare the efficiency of fluorophore coupling strategies for covalent DNA labelling. We compare a copper-catalyzed azide–alkyne cycloaddition reaction, amine to N-hydroxysuccin...
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| Publicado no: | Chem Sci |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Royal Society of Chemistry
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5436541/ https://ncbi.nlm.nih.gov/pubmed/28580113 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6sc04229e |
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