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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning st...
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| Vydáno v: | AMB Express |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Springer Berlin Heidelberg
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5433943/ https://ncbi.nlm.nih.gov/pubmed/28510221 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-017-0386-1 |
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