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A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level
Analysis of RNA dynamics and localization at the single-molecule level in living cells has been predominantly achieved by engineering target RNAs with large insertions of tandem repeat sequences that are bound by protein-based or oligonucleotide-based fluorescent probes. Thus, individual RNAs are ta...
Shranjeno v:
| izdano v: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Nature Publishing Group UK
2017
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5431543/ https://ncbi.nlm.nih.gov/pubmed/28484218 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-01740-1 |
| Oznake: |
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