Enhancing the throughput and multiplexing capabilities of next generation sequencing for efficient implementation of pooled shRNA and CRISPR screens

Next generation sequencing is becoming the method of choice for functional genomic studies that use pooled shRNA or CRISPR libraries. A key challenge in sequencing these mixed-oligo libraries is that they are highly susceptible to hairpin and/or heteroduplex formation. This results in polyclonal, lo...

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Publicado no:Sci Rep
Main Authors: Islam, Md. Fahmid, Watanabe, Atsushi, Wong, Lai, Lazarou, Conor, Vizeacoumar, Frederick S., Abuhussein, Omar, Hill, Wayne, Uppalapati, Maruti, Geyer, C. Ronald, Vizeacoumar, Franco J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5430825/
https://ncbi.nlm.nih.gov/pubmed/28432350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-01170-z
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