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Low-cost, Low-bias and Low-input RNA-seq with High Experimental Verifiability based on Semiconductor Sequencing

Low-input RNA-seq is powerful to represent the gene expression profiles with limited number of cells, especially when single-cell variations are not the aim. However, pre-amplification-based and molecule index-based library construction methods boost bias or require higher throughput. Here we demons...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Mai, Zhibiao, Xiao, Chuanle, Jin, Jingjie, Zhang, Gong
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5430657/
https://ncbi.nlm.nih.gov/pubmed/28432352
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-01165-w
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