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Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for creation of mutant mice with mutations mirroring those in human patients. Various methods have been developed for this purpose, including deletions, inversions, and tr...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Kato, Tomoko, Hara, Satoshi, Goto, Yuji, Ogawa, Yuya, Okayasu, Haruka, Kubota, Souichirou, Tamano, Moe, Terao, Miho, Takada, Shuji
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5427885/
https://ncbi.nlm.nih.gov/pubmed/28246396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-00140-9
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