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Transfer of a yeast artificial chromosome carrying human DNA from Saccharomyces cerevisiae into mammalian cells.

To test the feasibility of transferring yeast artificial chromosomes (YACs) into mammalian cells, we modified a YAC that carries approximately 450 kilobases (kb) of human DNA, by inserting a neomycin-resistance gene. Saccharomyces cerevisiae cells carrying this YAC were fused by polyethylene glycol...

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Main Authors: Pachnis, V, Pevny, L, Rothstein, R, Costantini, F
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC54271/
https://ncbi.nlm.nih.gov/pubmed/2195548
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