Near-Membrane Refractometry Using Supercritical Angle Fluorescence

Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet truly quantitative TIRF remains problematic. One unknown hampering the interpretation of evanescent-wave excited fluorescen...

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Bibliographic Details
Published in:Biophys J
Main Authors: Brunstein, Maia, Roy, Lopamudra, Oheim, Martin
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2017
Subjects:
Cell Biophysics
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5425786/
https://ncbi.nlm.nih.gov/pubmed/28494964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.03.008
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