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Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf Protoplasts

Freshly prepared spinach leaf protoplasts were gently ruptured by mechanical shearing followed by sucrose density gradient centrifugation to separate constituent cell organelles. The isolation of intact Class I chloroplasts (d = 1.21) in high yield, well separated from peroxisomes and mitochondria,...

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Sábháilte in:
Sonraí bibleagrafaíochta
Foilsithe in:Plant Physiol
Príomhchruthaitheoirí: Nishimura, Mikio, Graham, Douglas, Akazawa, Takashi
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Oxford University Press 1976
Ábhair:
Rochtain ar líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC542237/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16659669/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.58.3.309
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