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Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf Protoplasts

Freshly prepared spinach leaf protoplasts were gently ruptured by mechanical shearing followed by sucrose density gradient centrifugation to separate constituent cell organelles. The isolation of intact Class I chloroplasts (d = 1.21) in high yield, well separated from peroxisomes and mitochondria,...

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Bibliografische gegevens
Hoofdauteurs: Nishimura, Mikio, Graham, Douglas, Akazawa, Takashi
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1976
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC542237/
https://ncbi.nlm.nih.gov/pubmed/16659669
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