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Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf Protoplasts

Freshly prepared spinach leaf protoplasts were gently ruptured by mechanical shearing followed by sucrose density gradient centrifugation to separate constituent cell organelles. The isolation of intact Class I chloroplasts (d = 1.21) in high yield, well separated from peroxisomes and mitochondria,...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Plant Physiol
Egile Nagusiak: Nishimura, Mikio, Graham, Douglas, Akazawa, Takashi
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1976
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC542237/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16659669/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.58.3.309
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