Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf Protoplasts
Freshly prepared spinach leaf protoplasts were gently ruptured by mechanical shearing followed by sucrose density gradient centrifugation to separate constituent cell organelles. The isolation of intact Class I chloroplasts (d = 1.21) in high yield, well separated from peroxisomes and mitochondria,...
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| Publicado no: | Plant Physiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1976
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC542237/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16659669/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.58.3.309 |
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