Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.
We have developed a procedure that allows the detection of polymerase chain reaction (PCR) products derived from a single target DNA molecule in a human sperm without using radioactive probes. With this method, three genetic loci present in a single sperm can be amplified simultaneously. The amplifi...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54160/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1972276/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.12.4580 |
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