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Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

We have developed a procedure that allows the detection of polymerase chain reaction (PCR) products derived from a single target DNA molecule in a human sperm without using radioactive probes. With this method, three genetic loci present in a single sperm can be amplified simultaneously. The amplifi...

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Detaylı Bibliyografya
Yayımlandı:Proc Natl Acad Sci U S A
Asıl Yazarlar: Li, H, Cui, X, Arnheim, N
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: National Academy of Sciences 1990
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54160/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1972276/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.12.4580
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