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Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

We have developed a procedure that allows the detection of polymerase chain reaction (PCR) products derived from a single target DNA molecule in a human sperm without using radioactive probes. With this method, three genetic loci present in a single sperm can be amplified simultaneously. The amplifi...

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Detalhes bibliográficos
Main Authors: Li, H, Cui, X, Arnheim, N
Formato: Artigo
Idioma:Inglês
Publicado em: 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC54160/
https://ncbi.nlm.nih.gov/pubmed/1972276
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