A One-Step PCR-Based Assay to Evaluate the Efficiency and Precision of Genomic DNA-Editing Tools

Despite rapid progress, many problems and limitations persist and limit the applicability of gene-editing techniques. Making use of meganucleases, TALENs, or CRISPR/Cas9-based tools requires an initial step of pre-screening to determine the efficiency and specificity of the designed tools. This step...

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發表在:Mol Ther Methods Clin Dev
Main Authors: Germini, Diego, Bou Saada, Yara, Tsfasman, Tatiana, Osina, Kristina, Robin, Chloé, Lomov, Nikolay, Rubtsov, Mikhail, Sjakste, Nikolajs, Lipinski, Mar≿, Vassetzky, Yegor
格式: Artigo
語言:Inglês
出版: American Society of Gene & Cell Therapy 2017
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Original Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5415314/
https://ncbi.nlm.nih.gov/pubmed/28480303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtm.2017.03.001
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