Refolding of Escherichia coli dihydrofolate reductase: sequential formation of substrate binding sites.
The kinetics of refolding of Escherichia coli dihydrofolate reductase (EC 1.5.1.3) have been examined upon dilution of unfolded enzyme in 4.5 M urea to 1.29 M urea in 0.02 M phosphate buffer (pH 7.2) at 10 degrees C. Changes in the intrinsic protein fluorescence on refolding are characterized by fou...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1990
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54124/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2191290/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.12.4413 |
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