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Refolding of Escherichia coli dihydrofolate reductase: sequential formation of substrate binding sites.

The kinetics of refolding of Escherichia coli dihydrofolate reductase (EC 1.5.1.3) have been examined upon dilution of unfolded enzyme in 4.5 M urea to 1.29 M urea in 0.02 M phosphate buffer (pH 7.2) at 10 degrees C. Changes in the intrinsic protein fluorescence on refolding are characterized by fou...

Täydet tiedot

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Bibliografiset tiedot
Päätekijä: Frieden, C
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1990
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC54124/
https://ncbi.nlm.nih.gov/pubmed/2191290
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