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A method for labeling proteins with tags at the native genomic loci in budding yeast

Fluorescent proteins and epitope tags are often used as protein fusion tags to study target proteins. One prevailing technique in the budding yeast Saccharomyces cerevisiae is to fuse these tags to a target gene at the precise chromosomal location via homologous recombination. However, several limit...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Wang, Qian, Xue, Huijun, Li, Siqi, Chen, Ying, Tian, Xuelei, Xu, Xin, Xiao, Wei, Fu, Yu Vincent
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5411076/
https://ncbi.nlm.nih.gov/pubmed/28459859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0176184
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