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Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.

We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This technique uses a new, small P transposable element (the Wee-P) that is composed primarily of the green fluorescent protein (GFP) sequence flanked by consensus splice acceptor and splice donor sequences. We...

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Autors principals: Clyne, Peter J, Brotman, Jennie S, Sweeney, Sean T, Davis, Graeme
Format: Artigo
Idioma:Inglês
Publicat: 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1462835/
https://ncbi.nlm.nih.gov/pubmed/14668392
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