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Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
We describe a PCR- and homologous recombination-based system for generating targeted mutations in histone genes in budding yeast cells. The resulting mutant alleles reside at their endogenous genomic sites and no exogenous DNA sequences are left in the genome following the procedure. Since in haploi...
Tallennettuna:
| Julkaisussa: | J Vis Exp |
|---|---|
| Päätekijät: | , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
MyJove Corporation
2017
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5409331/ https://ncbi.nlm.nih.gov/pubmed/28190067 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55263 |
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