Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

Acute neuronal tissue preparations, brain slices and retinal wholemount, can usually only be maintained for 6 - 8 h following dissection. This limits the experimental time, and increases the number of animals that are utilized per study. This limitation specifically impacts protocols such as calcium...

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Vydáno v:J Vis Exp
Hlavní autoři: Cameron, Morven A., Kekesi, Orsolya, Morley, John W., Bellot-Saez, Alba, Kueh, Sindy, Breen, Paul, van Schaik, André, Tapson, Jonathan, Buskila, Yossi
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2017
Témata:
Neuroscience
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408972/
https://ncbi.nlm.nih.gov/pubmed/28287542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55396
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