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Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
Acute neuronal tissue preparations, brain slices and retinal wholemount, can usually only be maintained for 6 - 8 h following dissection. This limits the experimental time, and increases the number of animals that are utilized per study. This limitation specifically impacts protocols such as calcium...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5408972/ https://ncbi.nlm.nih.gov/pubmed/28287542 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55396 |
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