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Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

The Xenopus oocyte as a heterologous expression system for proteins, was first described by Gurdon et al.(1) and has been widely used since its discovery (References 2 - 3, and references therein). A characteristic that makes the oocyte attractive for foreign channel expression is the poor abundance...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Maldifassi, Maria C., Wongsamitkul, Nisa, Baur, Roland, Sigel, Erwin
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5408650/
https://ncbi.nlm.nih.gov/pubmed/28117773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55034
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