Escherichia coli thymidylate synthase: amino acid substitutions by suppression of amber nonsense mutations.

By using site-directed oligonucleotide mutagenesis, amber nonsense stop codons (5'-TAG-3') have been introduced at 20 sites in the Escherichia coli thymidylate synthase gene. By transforming the thyA mutant plasmids into 13 strains, each of which harbor different amber suppressor tRNAs, we were able...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Michaels, M L, Kim, C W, Matthews, D A, Miller, J H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54023/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2187197/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.10.3957
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