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Escherichia coli thymidylate synthase: amino acid substitutions by suppression of amber nonsense mutations.

By using site-directed oligonucleotide mutagenesis, amber nonsense stop codons (5'-TAG-3') have been introduced at 20 sites in the Escherichia coli thymidylate synthase gene. By transforming the thyA mutant plasmids into 13 strains, each of which harbor different amber suppressor tRNAs, we...

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書誌詳細
主要な著者: Michaels, M L, Kim, C W, Matthews, D A, Miller, J H
フォーマット: Artigo
言語:Inglês
出版事項: 1990
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC54023/
https://ncbi.nlm.nih.gov/pubmed/2187197
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