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Escherichia coli thymidylate synthase: amino acid substitutions by suppression of amber nonsense mutations.

By using site-directed oligonucleotide mutagenesis, amber nonsense stop codons (5'-TAG-3') have been introduced at 20 sites in the Escherichia coli thymidylate synthase gene. By transforming the thyA mutant plasmids into 13 strains, each of which harbor different amber suppressor tRNAs, we...

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Bibliografiske detaljer
Main Authors: Michaels, M L, Kim, C W, Matthews, D A, Miller, J H
Format: Artigo
Sprog:Inglês
Udgivet: 1990
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC54023/
https://ncbi.nlm.nih.gov/pubmed/2187197
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