Evidence for Preferential Genotyping of a Minority Human Immunodeficiency Virus Population Due to Primer-Template Mismatching during PCR-Based Amplification

Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, m...

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Detalhes bibliográficos
Main Authors: Martinez-Picado, Javier, Morales-Lopetegi, Kristina, Villena, Cristina, Gutiérrez, Carolina, Izquierdo, Nuria, Marfil, Silvia, Clotet, Bonaventura, Ruiz, Lidia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC540107/
https://ncbi.nlm.nih.gov/pubmed/15635008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.1.436-438.2005
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