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The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studies.

We investigated the effects of internal primer-template mismatches on the efficiency of reverse transcription and PCR amplification. As models, RNA transcripts representative of different HIV-1 group M subtypes were evaluated with a previously described gag primer pair system. We observed that the p...

詳細記述

保存先:
書誌詳細
主要な著者: Christopherson, C, Sninsky, J, Kwok, S
フォーマット: Artigo
言語:Inglês
出版事項: 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC146455/
https://ncbi.nlm.nih.gov/pubmed/9016609
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