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The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studies.

We investigated the effects of internal primer-template mismatches on the efficiency of reverse transcription and PCR amplification. As models, RNA transcripts representative of different HIV-1 group M subtypes were evaluated with a previously described gag primer pair system. We observed that the p...

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Udgivet i:Nucleic Acids Res
Principais autores: Christopherson, C, Sninsky, J, Kwok, S
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146455/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016609/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.3.654
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