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A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.

The complex between double-stranded DNA and ethidium homodimer (5,5'-diazadecamethylene)bis(3,8-diamino-6-phenylphenanthridini um) cation, formed at a ratio of 1 homodimer per 4 or 5 base pairs, is stable in agarose gels under the usual conditions for electrophoresis. This unusual stability all...

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Bibliografske podrobnosti
Main Authors: Glazer, A N, Peck, K, Mathies, R A
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC54001/
https://ncbi.nlm.nih.gov/pubmed/2339125
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