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Dramatic Improvement of CRISPR/Cas9 Editing in Candida albicans by Increased Single Guide RNA Expression

The clustered regularly interspaced short palindromic repeat system with CRISPR-associated protein 9 nuclease (CRISPR/Cas9) has emerged as a versatile tool for genome editing in Candida albicans. Mounting evidence from other model systems suggests that the intracellular levels of single guide RNA (s...

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Bibliographic Details
Published in:mSphere
Main Authors: Ng, Henry, Dean, Neta
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5397569/
https://ncbi.nlm.nih.gov/pubmed/28435892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00385-16
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