3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events...

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Vydáno v:J Cell Sci
Hlavní autoři: Russell, Matthew R. G., Lerner, Thomas R., Burden, Jemima J., Nkwe, David O., Pelchen-Matthews, Annegret, Domart, Marie-Charlotte, Durgan, Joanne, Weston, Anne, Jones, Martin L., Peddie, Christopher J., Carzaniga, Raffaella, Florey, Oliver, Marsh, Mark, Gutierrez, Maximiliano G., Collinson, Lucy M.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Company of Biologists Ltd 2017
Témata:
Tools and Techniques
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5394779/
https://ncbi.nlm.nih.gov/pubmed/27445312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.188433
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