3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events...

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Bibliografske podrobnosti
izdano v:J Cell Sci
Main Authors: Russell, Matthew R. G., Lerner, Thomas R., Burden, Jemima J., Nkwe, David O., Pelchen-Matthews, Annegret, Domart, Marie-Charlotte, Durgan, Joanne, Weston, Anne, Jones, Martin L., Peddie, Christopher J., Carzaniga, Raffaella, Florey, Oliver, Marsh, Mark, Gutierrez, Maximiliano G., Collinson, Lucy M.
Format: Artigo
Jezik:Inglês
Izdano: The Company of Biologists Ltd 2017
Teme:
Tools and Techniques
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5394779/
https://ncbi.nlm.nih.gov/pubmed/27445312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.188433
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