3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events...

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Bibliografiske detaljer
Udgivet i:J Cell Sci
Main Authors: Russell, Matthew R. G., Lerner, Thomas R., Burden, Jemima J., Nkwe, David O., Pelchen-Matthews, Annegret, Domart, Marie-Charlotte, Durgan, Joanne, Weston, Anne, Jones, Martin L., Peddie, Christopher J., Carzaniga, Raffaella, Florey, Oliver, Marsh, Mark, Gutierrez, Maximiliano G., Collinson, Lucy M.
Format: Artigo
Sprog:Inglês
Udgivet: The Company of Biologists Ltd 2017
Fag:
Tools and Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5394779/
https://ncbi.nlm.nih.gov/pubmed/27445312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.188433
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