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DNA-free RNA preparations from mycobacteria

BACKGROUND: To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. RESULTS: After sonication of RNA samples from Mycob...

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Detalhes bibliográficos
Main Authors: Stephan, Joachim, Bail, Johannes G, Titgemeyer, Fritz, Niederweis, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC539233/
https://ncbi.nlm.nih.gov/pubmed/15571628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-4-45
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